Fig. 2.

Endogenous levels of CD271 influence tumor growth. a, b Melanoma cell lines with different levels of CD271 (CD271 low = M010817; CD271 high = M050829 were stably transduced with lentiviral backbones expressing infrared fluorescent protein (iRFP) and injected in to Nude mice. The growth of the xenografts was assessed by IVIS a and macroscopically after euthanasia b. c H&E staining and CD271 immunofluorescence of tumors formed from low and high CD271-expressing melanoma cells. Scale bars upper panel 2 mm, scale bars lower panel 50 µm. d, e qRT-PCR and western blot of the CD271 low melanoma cell line (M010817) treated with TGFβ1 ligand (5 ng/ml) at different time points. (FN: Fibronectin; CDH2: N-cadherin; β-act.: beta actin). N = 3, P values CD271 > 0.05 and ≤0.001, P value FN ≤ 0.001, P value N-cadh. ≤0.001. Error bars indicate S.D