Fig. 8.

CD271-mediated upregulation of cholesterol synthesis genes is TrkA dependent and regulates cell adhesion. a–i qRT-PCR for cholesterol synthesis genes in CMVTOEV and CMVTOCD271 cells transfected with siCtrl or siTrkA and 24 h later treated with doxycycline (1 µg/ml) for 24 h. N = 3 for each condition. P values > 0.05 = n.s., P values ≤ 0.05 = *, P values ≤ 0.01 = **, P values ≤ 0.001 = ***. j Crystal violet staining of cells in suspension reattached on FN plates after 24 h of combinatorial treatment with doxycycline and vehicle (DMSO), Lovastatin (2.5 µM) or MF-438 (15 µM). k Quantification of amount of cells in adherent and suspension fractions after combinatorial cell treatment with doxycycline and vehicle (DMSO), Lovastatin or MF-438. N = 3 for each condition. P values > 0.05 = n.s., P values ≤ 0.05 = *. Error bars for a–i and k indicate S.D. All experiments done with cell line M010817