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. 2017 Dec 7;7:17126. doi: 10.1038/s41598-017-17067-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Study design and neutralizing antibody testing. (A) After intramuscular inoculation of ZIKV at day 0, samples (serum, stool, urine, saliva, and semen) are collected at predesignated time points. (B) Longitudinally collected serum samples from inoculated marmosets were tested at predesignated time points for ZIKV-specific neutralizing antibodies using a PRNT (plaque reduction neutralization test). The antibody titer as determined by PRNT at a given time point is shown below the icon. Three of the 5 marmosets were sacrificed after approximately 1 month (denoted by a red “X”) to assess viral persistence in tissues.