Table 1.
Cell culture of ZIKV from infected body fluids. Abbreviations: CPE, cytopathic effect; RT-PCR, reverse-transcriptase polymerase chain reaction; Ct, cycle threshold.
| Primate ID | Collection day post-infection | Sample type | Volume of inoculum (μL) | Viral RNA copies of inoculum | Passage number | CPE present* | RT-PCR [Ct] of culture supernatant |
|---|---|---|---|---|---|---|---|
| 1 | 3 | saliva | 100 | 1.24E + 03 | 0 | ++ | + [24.0] |
| 1 | 3 | serum | 20 | 4.10E + 03 | 0 | ++ | + [18.3] |
| 2 | 3 | serum | 20 | 2.08E + 03 | 0 | ++ | + [18.1] |
| 3 | 3 | serum | 20 | 4.14E + 03 | 0 | ++ | + [19.4] |
| 4 | 3 | serum | 20 | 4.00E + 03 | 0 | ++ | + [18.0] |
| 1 | 7 | saliva | 100 | 3.88E + 04 | 0 | ++ | + [18.2] |
| 2 | 7 | saliva | 100 | 1.41E + 03 | 0 | ++ | + [21.7] |
| 3 | 7 | saliva | 100 | 1.92E + 02 | 0 | ++ | + [22.8] |
| 4 | 7 | saliva | 100 | 2.40E + 03 | 0 | ++ | + [24.2] |
| 4 | 7 | urine | 200 | 1.92E + 04 | 1# | — | — |
| 1 | 13 | urine | 200 | 7.52E + 02 | 1# | — | — |
| 4 | 13 | urine | 200 | 7.08E + 03 | 1# | — | — |
| 1 | 14 | saliva | 200 | 6.86E + 01 | 0 | — | — |
| 4 | 14 | saliva | 200 | 1.57E + 03 | 0 | — | — |
| 4 | 14 | semen | 400 | 1.91E + 03 | 0 | — | — |
| 4 | 14 | semen swab | 400 | 5.60E + 02 | 0 | — | — |
* −, no CPE, +, mild-moderate CPE (<0–25%), ++, heavy CPE (>25%), reported from duplicate wells; cells were monitored daily for up to 14 days or until CPE was observed
#atypical cytotoxicity observed at passage 0, so cell culture supernatants passaged once in Vero cells.