Fig. 5.

FOXC2 is a transcriptional repressor of p120-catenin. a Upon FOXC2 knockdown, p120-catenin expression at both the transcript and protein level was determined by qRT-PCR and Western blot respectively. Densitometry analysis for p120-catenin is shown. b The effect of FOXC2 knockdown on p120-catenin promoter activity was evaluated using a p120-catenin promoter luciferase reporter construct. c Expression of FOXC2 and p120-catenin protein upon PKCα knockdown in MCF7 cells was determined by Western blots. d FOXC2 binding to the p120-catenin promoter was determined by ChIP assay. e FOXC2 binding to the p120-catenin promoter with PKCα knockdown was determined by ChIP assay. Experiments were done in two representative cell lines in MCF7/PKCα (endocrine resistant) and HCC1937 (basal A TNBC). All blot images are representative of at least three independent biological replicates. β-actin was used as the loading control. Graphs represent the SEM of at least three independent biological replicates. Significance was determined by student t-test (a, b, d) and two-way ANOVA, followed by Tukey’s test (e). *, P < 0.05 **, P < 0.01 ****, P < 0.0001