Table 1.

Characteristics and sensitivity of colorectal cancer cell lines to valproic acid and 5′-deoxy-5-fluorouridine (5′-DFUR)

Cell lines	p53	KRAS	B-RAF	PI3KCA	IC50 96 h VPA, mM ± SD	IC50 96 h 5′-DFUR, μM ± SD
HT29	mut (R273H)	wt	mut	mut	3.35 ± 0.49	9.88 ± 2.14
SW620	mut (R273H)	mut (G12 V)	wt	wt	2.01 ± 0.52	35.31 ± 5.95
HCT-116	wt	mut (G13D)	wt	mut	1.41 ± 0.19	2.16 ± 0.43
HCT-116 p53−/−	null	mut (G13D)	wt	mut	2.35 ± 0.43	3.41 ± 0.68

IC₅₀ values were computed at 96 h of treatment (mean ± standard deviation (SD) from at least three separate experiments performed in quadruplicates); (mut is for mutant and wt for wild-type)