Fig. 3.

Visualizing the staining pattern in 3D by FIB-SEM. The figure panels show cells expressing MiD51-APEX2 (A), MiD49-APEX2 (B), APEX2-Mff (C), APEX2-Fis1 (D), and APEX2-Mfn2 (E). The left columns display the representative SEM micrographs from each volume of EM data, and the right columns display the 3D rendering of boxed areas of the left with the yellow spheres for mitochondria and purple layers for APEX2-induced EM contrast. The labels in the top left corner of each micrograph show the relevant position between the target protein and the tag as well as their relevant sizes. Scale bars, 5 μm