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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Mol Microbiol. 2017 Nov 2;106(5):793–814. doi: 10.1111/mmi.13847

Fig 4.

Fig 4

ΔkhpB mutants phenocopy ΔkhpA mutants exactly. WT (IU1824), ΔkhpA (IU9036), ΔkhpB (IU10592), and ΔkhpA ΔkhpB (IU10596) mutants were compared. A. Growth curves in BHI broth. Doubling times (DT) were calculated from 3 independent growth curves for cells growing exponentially (OD620=0.03–0.3). B. Representative phase-contrast images of cells in early exponential phase. Scale bar = 1 μm. C. Relative transcript amounts of spd_1874 and spd_0104 of the WalRK regulon in ΔkhpA, ΔkhpB, and ΔkhpA ΔkhpB mutants compared to WT, determined by qRT-PCR as described in Experimental procedures. Averages ± SE are shown from 3 independent experiments. D. Box-and-whisker plots (whiskers, 5 and 95 percentile) of cell length, width, aspect ratio (length/width), and relative cell volume of WT, ΔkhpA, ΔkhpB, and ΔkhpA ΔkhpB cells; >100 cells from exponential cultures were measured for each strain in 2 independent experiments. For C and D, P values were calculated as for Figure 2. ns, not significantly different.