Fig. 5.

Disease symptoms of Botrytis cinerea (stain SAS56 containing pCutGUS) on inoculated leaves of cv Desiree and StDND1 well-silenced potato plants at 48 h post inoculation. Left panel (a and b) photos of B. cinerea infected leaves of cv Desiree and StDND1 well-silenced plants. Yellow arrows point to necrotic spots occurring at the inoculation sites. Right panel (c) GUS and DAB staining of leaf samples with inoculation sites from cv Desiree and StDND1 well-silenced potato plants after B. cinerea inoculation (IN) or mock treatment (PDB). GUS stains hyphae of B. cinerea as blue spots at inoculation sites. DAB stains the H₂O₂ accumulation as brownish areas at inoculation sites. IN, inoculated; PDB, Potato Dextrose Broth medium; GUS, beta-glucuronidase; DAB, 3,3′-diaminobenzidine