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. Author manuscript; available in PMC: 2017 Dec 7.
Published in final edited form as: Eur J Med Chem. 2016 Jun 29;122:770–785. doi: 10.1016/j.ejmech.2016.06.045

Figure 8.

Figure 8

A: MTT cell viability assays conducted on 2–7 using the Colon-26 carcinoma cell line were irradiated at a fluence of 4 J/cm2 after 24 h incubation with the compounds. MTT was done at 48 h post-irradiation. The medium linkers 3 and 6 were more efficacious than the long and short linked compounds. The in vitro phototoxicity followed the order medium > long > short linked conjugates when their EC50 values were compared. B: In initial study the PDT efficacy of the conjugates was assessed at different drug doses, and the same light dose. The optimal drug dose was determined and the comparative efficacy of other conjugates was determined at the similar treatment parameters (drug dose, 1.5 µmol/kg, light dose, 128 J/cm2, 14 mW/cm2) following the animal protocol approved by the institute’s IACUC committee. The mice with no tumor re-growth at day 60 were listed in “complete cure” category. For in vitro/in vivo experiments, a tunable dye laser (Spectra-Physics, Mt. View, CA) was used as a light source.