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. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: Cancer Lett. 2017 Sep 28;411:136–149. doi: 10.1016/j.canlet.2017.09.033

Fig. 2.

Fig. 2

Effect of GLI antagonists JK184, HPI-1, HPI-4 and GANT61 on SUM149, MDA-MB-231 and SUM159 colony formation. For colony formation, cells were seeded in 6-well plates, treated with the indicated compounds for 5–14 days, fixed, colonies stained and counted. (A) Representative images for SUM149 and MDA-MB-231 colonies stained with crystal violet. A minimum of two independent experiments were carried out. (B) SUM149 and MDA-MB-231 percent colony formation relative to vehicle. (C) Representative images for SUM159 colonies and percent colony formation relative to vehicle. Data represents mean ± SD percentage of colonies relative to vehicle/untreated for a minimum of three wells. One-way ANOVA with Tukey’s post-test (*p<0.05, **p<0.005, ***p<0.0005 and ****p<0.0001; significantly different from respective controls).