Table 1.
IC50 values for GLI antagonists tested in C3H10T1/2 hedgehog functional assay and in Hoechst proliferation assay.
| IC50 (µM) (AP activity)a |
IC50 (µM) (Proliferation)b |
|||
|---|---|---|---|---|
| C3H10T1/2 | SUM149 | MDA-MB-231 | SUM159 | |
| HPI-1 | 1.1 | 29 | > 25 | 20.5 |
| HPI-2 | ||||
| HPI-3 | 0.7 | |||
| HPI-4 | 50% @ 10 µM | 15.9 | 6.2 | No curve |
| GANT58 | >10 | >25 | ||
| GANT61 | 3.7 | 6.3 | 5.6 | 6.1 |
| JK184 | 0.02 | 0.01 | 0.08 | 0.05 |
| Cyclopamine | 0.2 |
a
GLI antagonists were assayed in dose response in the C3H10T1/2 Hh functional assay in the presence of 2 µg/ml Hh protein, and AP activity measured after 5 days.
b
GLI antagonists were tested in dose response in Hoechst assay as described in Materials and Methods. IC50 values were determined from a minimum of three independent experiments.