Table 2.
IC50 (µM)a | ||||
---|---|---|---|---|
SUM149 | MDA-MB-231 | SUM159 | ||
Stauropsorine | Spheroid Area | 0.011 | No curve | 0.007 |
Spheroid YOYO-1 | 0.021 | No curve | 0.065 | |
GANT61 | Spheroid Area | 18 | 16.7 | 22.6 |
Spheroid YOYO-1 | 25.9 | No curve | 37.8 |
GLI antagonists were tested in dose response in 3D tumor spheroid assay, stained with Hoechst and YOYO-1 and imaged as described in Materials and Methods. IC50 values were determined by non-linear regression in Graphpad Prism 6. A minimum of three independent experiments were conducted.