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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Cell Microbiol. 2017 Oct 4;19(12):10.1111/cmi.12767. doi: 10.1111/cmi.12767

FIGURE 2. rta3Δ/Δ displays increased internalization of NBD-PC.

FIGURE 2

Strains were grown to an OD600 of 1, labelled with 5 µM NBD-labelled PC and co-stained with 20 nM MitoTracker™ Red (MTR) for 45 min at 30 °C. Cells were washed, kept in fresh SDC media for 30 min at 30 °C, washed with ice cold SC-azide three times and visualized by confocal microscopy. Values on the right indicate mean fluorescence intensity (MFI) of internalized NBD-PC in all strains measured by flow cytometry (*P < 0.01, Student’s t-test). Data represent the mean ± S.D of three independent experiments. Approximately 200 cells were visualized using confocal microscopy. m denotes mitochondria.