Antibodies |
|
Rat monoclonal anti-CD45 [30-F11] |
BD Biosciences |
Cat#: 553076, RRID:AB_394606
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Rat monoclonal anti-F4/80 [Cl:A3-1] |
Bio-Rad |
Cat#: MCA497R, RRID:AB_323279
|
Goat polyclonal anti-MMR/CD206 |
R&D Systems |
Cat#: AF2535, RRID:AB_2063012
|
Rabbit monoclonal anti-CD3 [SP7] |
ThermoFisher |
Cat#: RM-9107-RQ |
Rabbit polyclonal anti-CD274/PD-L1 |
Abcam |
Cat#: ab58810, RRID:AB_940872
|
Rabbit monoclonal anti-Ki67 [SP6] |
ThermoFisher |
Cat#: RM-9106-S1, RRID:AB_149792
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Rat anti-CD31/PECAM1 [MEC13.3] |
BD Biosciences |
Cat#: 550274, RRID:AB_393571
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Rat monoclonal anti-Pan-Endothelial cell antigen/PVLAP [MECA32] |
BD Biosciences |
Cat#: 550563, RRID:AB_393754
|
Mouse monoclonal anti-VEGF:VEGFR2 (GV39M) |
EastCoast Bio |
Cat#: CD301 |
Rabbit monoclonal anti-IL23p19 |
Abcam |
Cat#: ab45420, RRID:AB_2124515
|
Goat polyclonal anti-CCL9/10/MIP1γ |
R&D Systems |
Cat#: AF-463, RRID:AB_2071817
|
Rabbit monoclonal anti-TTF1 [EP1584Y] |
Abcam |
Cat#: ab76013, RRID:AB_1310784
|
Mouse monoclonal anti-TTF1 [8G7G3/1] |
Abcam |
Cat#: ab72876, RRID:AB_1271363
|
Mouse monoclonal anti-Hypoxyprobe [4.3.11.3] |
Hypoxyprobe |
Cat#: HP1-100 |
Polyclonal goat anti-Rae-1 Pan-specific |
R&D Systems |
Cat#: AF1136, RRID:AB_2238016
|
Rat monoclonal anti-MHC1 [ER-HR52] |
Novus Biologicals |
Cat#: NB100-64952, RRID:AB_964497
|
Rat monoclonal anti-CD45R/B220 [RA3-6B2] |
ThermoFisher |
Cat#: MA1-70098, RRID:AB_1072441
|
Rat anti-CD335/NKp46 [29A1.4] |
Biolegend |
Cat#: 137601, RRID:AB_10551441
|
Fluorescein-labeled Lycopersicon Esculentum (Tomato) Lectin |
Vector Labs |
Cat#: FL-1171, RRID:AB_2307440
|
Rhodamine-labeled Ricinus communis Agglutinin I |
Vector Labs |
Cat#: RL-1082, RRID:AB_2336710
|
LEAF-purified Rat anti-mouse CD274/PD-L1 [10F.9G2] |
Biolegend |
Cat#: 124309, RRID:AB_2291192
|
LEAF-purified Rat IgG2b, κ Isotype control [RTK4530] |
Biolegend |
Cat#: 400637, RRID:AB_2086803
|
LEAF-purified Mouse anti-mouse IL23p19 [MMp19B2] |
Biolegend |
Cat#: 513806, RRID:AB_2562073
|
LEAF-purified Mouse IgG2b, κ Isotype control [MPC-11] |
Biolegend |
Cat#: 400339 |
Goat polyclonal anti-mouse CCL9/10/MIP1γ |
R&D Systems |
Cat#: AF463, RRID:AB_2071817
|
Goat IgG control |
R&D Systems |
Cat#: AB-108-C, RRID:AB_354267
|
Rat anti-mouse CD4 [GK1.5] |
BioXcell |
Cat#: BE0003, RRID:AB_1107636
|
Rat anti-mouse CD8 [2.43] |
BioXcell |
Cat#: BE0061, RRID:AB_1125541
|
Rat IgG2b control [LTF2] |
BioXcell |
Cat#: BE0090, RRID:AB_1107780
|
Rabbit anti-mouse asialo-GM1 |
Wako Chemicals |
Cat#: 986-10001, RRID:AB_516844
|
Rabbit IgG control |
R&D Systems |
Cat#: AB-105-C, RRID:AB_354266
|
Rat anti-mouse CD3ε [145-2C11] |
eBioscience /ThermoFisher |
Cat#: 17-0031, RRID:AB_469313
|
Rat anti-mouse CD8α [53-6.7] |
eBioscience /ThermoFisher |
Cat#: 12-0081, RRID:AB_465529
|
Rat anti-mouse CD4 [RM4-5] |
eBioscience /ThermoFisher |
Cat#: 14-0042, RRID:AB_467066
|
Rat anti-mouse CD4 [GK1.5] |
eBioscience /ThermoFisher |
Cat#: 11-0041, RRID:AB_464891
|
Rat anti-mouse CD45 [30-F11] |
eBioscience /ThermoFisher |
Cat#: 14-0451, RRID:AB_467252
|
Rat anti-mouse CD335/NKp46 [29A1.4] |
eBioscience /ThermoFisher |
Cat#: 11-3351, RRID:AB_1210843
|
|
Chemicals |
|
Tamoxifen |
Sigma-Aldrich |
Cat#: T5648 |
7-AAD Viability staining solution |
eBioscience |
Cat#: 00-6993-50 |
|
Critical Commercial Assays, Macrophage Isolation |
|
Mouse Inflammation Antibody Array - Membrane |
Abcam |
Cat#: ab133999 |
ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit (TUNEL) |
Millipore |
Cat#: S7160 |
High Capacity cDNA RT kit |
Applied Biosystems |
Cat#: 4374966 |
Fast Sybr Green |
Applied Biosystems |
Cat#: 4385612 |
RNeasy Plus Mini Kit |
QIAGEN |
Cat#: 74134 |
Anti-F4/80-biotin [REA126] |
Miltenyi Biotech |
Cat#: 130-101-893, RRID: AB_2651713
|
Anti-F4/80 Microbeads, Ultra Pure |
Miltenyi Biotech |
Cat#: 130-110-443 |
RNAscope 2.5 HD Reagent Kit-BROWN |
Advanced Cell Diagnostics |
Cat#: 322300 |
TSA Plus multi-fluorophore detection kit |
PerkinElmer |
Cat#: NEL760001KT |
|
Experimental Models: Organisms/Strains |
|
LSL-KRasG12D
|
Jackson et al., 2001 |
N/A |
Rosa26LSLMycERT2
|
Murphy et al., 2008 |
N/A |
|
Software and Algorithms |
|
FlowJo (version X) |
FlowJo |
RRID: SCR_008520; https://www.flowjo.com/
|
ImageJ/Fiji |
Open source |
RRID: SCR_003070 |
Graphpad Prism |
GraphPad Software |
RRID: SCR_002798; https://www.graphpad.com/scientific-software/prism/
|