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. 2017 Nov 30;171(6):1301–1315.e14. doi: 10.1016/j.cell.2017.11.013
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rat monoclonal anti-CD45 [30-F11] BD Biosciences Cat#: 553076, RRID:AB_394606
Rat monoclonal anti-F4/80 [Cl:A3-1] Bio-Rad Cat#: MCA497R, RRID:AB_323279
Goat polyclonal anti-MMR/CD206 R&D Systems Cat#: AF2535, RRID:AB_2063012
Rabbit monoclonal anti-CD3 [SP7] ThermoFisher Cat#: RM-9107-RQ
Rabbit polyclonal anti-CD274/PD-L1 Abcam Cat#: ab58810, RRID:AB_940872
Rabbit monoclonal anti-Ki67 [SP6] ThermoFisher Cat#: RM-9106-S1, RRID:AB_149792
Rat anti-CD31/PECAM1 [MEC13.3] BD Biosciences Cat#: 550274, RRID:AB_393571
Rat monoclonal anti-Pan-Endothelial cell antigen/PVLAP [MECA32] BD Biosciences Cat#: 550563, RRID:AB_393754
Mouse monoclonal anti-VEGF:VEGFR2 (GV39M) EastCoast Bio Cat#: CD301
Rabbit monoclonal anti-IL23p19 Abcam Cat#: ab45420, RRID:AB_2124515
Goat polyclonal anti-CCL9/10/MIP1γ R&D Systems Cat#: AF-463, RRID:AB_2071817
Rabbit monoclonal anti-TTF1 [EP1584Y] Abcam Cat#: ab76013, RRID:AB_1310784
Mouse monoclonal anti-TTF1 [8G7G3/1] Abcam Cat#: ab72876, RRID:AB_1271363
Mouse monoclonal anti-Hypoxyprobe [4.3.11.3] Hypoxyprobe Cat#: HP1-100
Polyclonal goat anti-Rae-1 Pan-specific R&D Systems Cat#: AF1136, RRID:AB_2238016
Rat monoclonal anti-MHC1 [ER-HR52] Novus Biologicals Cat#: NB100-64952, RRID:AB_964497
Rat monoclonal anti-CD45R/B220 [RA3-6B2] ThermoFisher Cat#: MA1-70098, RRID:AB_1072441
Rat anti-CD335/NKp46 [29A1.4] Biolegend Cat#: 137601, RRID:AB_10551441
Fluorescein-labeled Lycopersicon Esculentum (Tomato) Lectin Vector Labs Cat#: FL-1171, RRID:AB_2307440
Rhodamine-labeled Ricinus communis Agglutinin I Vector Labs Cat#: RL-1082, RRID:AB_2336710
LEAF-purified Rat anti-mouse CD274/PD-L1 [10F.9G2] Biolegend Cat#: 124309, RRID:AB_2291192
LEAF-purified Rat IgG2b, κ Isotype control [RTK4530] Biolegend Cat#: 400637, RRID:AB_2086803
LEAF-purified Mouse anti-mouse IL23p19 [MMp19B2] Biolegend Cat#: 513806, RRID:AB_2562073
LEAF-purified Mouse IgG2b, κ Isotype control [MPC-11] Biolegend Cat#: 400339
Goat polyclonal anti-mouse CCL9/10/MIP1γ R&D Systems Cat#: AF463, RRID:AB_2071817
Goat IgG control R&D Systems Cat#: AB-108-C, RRID:AB_354267
Rat anti-mouse CD4 [GK1.5] BioXcell Cat#: BE0003, RRID:AB_1107636
Rat anti-mouse CD8 [2.43] BioXcell Cat#: BE0061, RRID:AB_1125541
Rat IgG2b control [LTF2] BioXcell Cat#: BE0090, RRID:AB_1107780
Rabbit anti-mouse asialo-GM1 Wako Chemicals Cat#: 986-10001, RRID:AB_516844
Rabbit IgG control R&D Systems Cat#: AB-105-C, RRID:AB_354266
Rat anti-mouse CD3ε [145-2C11] eBioscience /ThermoFisher Cat#: 17-0031, RRID:AB_469313
Rat anti-mouse CD8α [53-6.7] eBioscience /ThermoFisher Cat#: 12-0081, RRID:AB_465529
Rat anti-mouse CD4 [RM4-5] eBioscience /ThermoFisher Cat#: 14-0042, RRID:AB_467066
Rat anti-mouse CD4 [GK1.5] eBioscience /ThermoFisher Cat#: 11-0041, RRID:AB_464891
Rat anti-mouse CD45 [30-F11] eBioscience /ThermoFisher Cat#: 14-0451, RRID:AB_467252
Rat anti-mouse CD335/NKp46 [29A1.4] eBioscience /ThermoFisher Cat#: 11-3351, RRID:AB_1210843

Chemicals

Tamoxifen Sigma-Aldrich Cat#: T5648
7-AAD Viability staining solution eBioscience Cat#: 00-6993-50

Critical Commercial Assays, Macrophage Isolation

Mouse Inflammation Antibody Array - Membrane Abcam Cat#: ab133999
ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit (TUNEL) Millipore Cat#: S7160
High Capacity cDNA RT kit Applied Biosystems Cat#: 4374966
Fast Sybr Green Applied Biosystems Cat#: 4385612
RNeasy Plus Mini Kit QIAGEN Cat#: 74134
Anti-F4/80-biotin [REA126] Miltenyi Biotech Cat#: 130-101-893, RRID: AB_2651713
Anti-F4/80 Microbeads, Ultra Pure Miltenyi Biotech Cat#: 130-110-443
RNAscope 2.5 HD Reagent Kit-BROWN Advanced Cell Diagnostics Cat#: 322300
TSA Plus multi-fluorophore detection kit PerkinElmer Cat#: NEL760001KT

Experimental Models: Organisms/Strains

LSL-KRasG12D Jackson et al., 2001 N/A
Rosa26LSLMycERT2 Murphy et al., 2008 N/A

Software and Algorithms

FlowJo (version X) FlowJo RRID: SCR_008520; https://www.flowjo.com/
ImageJ/Fiji Open source RRID: SCR_003070
Graphpad Prism GraphPad Software RRID: SCR_002798; https://www.graphpad.com/scientific-software/prism/