Fig. 1.

Geraniin exhibits protective effect against TBH-induced cytotoxicity in HepG2 cells. (A) HepG2 cells were treated with various concentrations of geraniin for 24 h, and the viability was measured by MTT assay. Data shown represent the mean values of three experiments ± SE. *P < 0.05 vs. control. (B) Cells were pretreated with different concentrations of geraniin (0, 1, 10, and 25 μM) for 2 hrs, followed by exposure to TBH (2 mM). Cell viability was assessed by MTT assay. Data shown represent the mean values of three experiments ± SE. *P < 0.05 vs. control. **P < 0.05 vs. TBH (2 mM). (C) Cell morphology was observed under inverted microscope. (D) Cell cycle analysis was done in presence and absence of geraniin (25 μM). (E) Inhibition of intracellular ROS generated and (F) lipid peroxidation on TBH treatment in presence of geraniin. Data shown represent the mean values of three experiments ± SE. *P < 0.05 vs. control. **P < 0.05 vs. TBH (2 mM).