Fig 2. GABA elicits Ca²⁺ influx transients in DCs.

(A) Representative pseudocolor micrographs of live cell Ca²⁺ imaging of DCs loaded with 2 μM Fluo-8H/AM as described in Materials and Methods. Continuous line indicates perfusion time of GABA (10 mM) and dashed line indicates perfusion time of ATP (50 μM). For each micrograph color scale indicate fluorescence intensity at indicated time point (min:sec). (B) Relative fluorescence intensity of DCs as in (A). The lines indicate perfusions of GABA (10 mM) after 3 min and ATP (50 μM) after 6.5 min, respectively. Displayed data are 3 representative traces from one experiment. (C) Relative fluorescence intensity of DCs as in (A) and (B). Displayed data are 5 superposed representative traces from the same experiment. Experiments were performed 3 times with similar results (n = 15–20 cells per experiment).