Fig 3. Exogenous GABA and Ca²⁺ receptor agonism reconstitutes DC hypermotility.

(A) Mass spectrometry analysis of DCs challenged with tachyzoites (PRU, MOI 3) for 16 h ± GABAergic inhibition (SC, 50 μM; SNAP, 50 μM). Cell supernatant was analyzed as indicated under Materials and Methods. Mod. R indicates modified Krebs-Ringer’s solution. Data are representative of 3 independent experiments. (B) Representative motility plot analyses of DCs incubated with tachyzoites (PTG, MOI 3) for 2 h with SC (50 μM), SNAP (50 μM), ± GABA (5 μM) or Bay K8644 (10 μM). (C) Compiled motility analysis of DCs under same conditions as in (B). Motility assays were performed as described under Materials and Methods. Data represent median velocities ± SD of 3 independent experiments. Asterisks indicate significant differences (*: p < 0.01, ns: p ≥ 0.05, Pairwise Wilcoxon rank-sum test, Holm correction).