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. 2017 Dec 7;12(12):e0189162. doi: 10.1371/journal.pone.0189162

Fig 7. TLa ablation resulted in larva of smaller size.

Fig 7

A) Construct design for generating a stable GFP transgenic line. 1.5kb upstream of the initiation codon of kctd15a was cloned upstream of GFP and inserted into the genome using Tol2. B) A dorsal maximum projection of an average representation of the transgenic line at 6 dpf (green) overlayed on vGLUT expression (gray) shows strong expression in the pallium region of the forebrain and torus lateralis in the midbrain. The superimposed heat map indicates the region missing in our kctd15 mutants. C) Growth measurements taken over the course of 5 weeks of non-ablated control larva (see methods; n = 39–55 per time point), control pallium ablated (n = 8 per time point), TLa ablated (n = 24–26 per time point) and kctd15 mutants (n = 28 per time point). At 6 dpf entire larval length was measured, at all other time points standard length (SL) was measured. See text for ANOVA results. For post-hoc t-tests: (a) indicates p < 0.05 for torus lateralis (TLa) ablated compared to intact control or pallium ablated controls. (b) indicates p < 0.05 for TLa compared to kctd15 mutants.