Fig 2. Determination of phnW expression levels in wild-type and mutant strains when exposed to t-BOOH.

All bacteria which contained a phnW-lacZ transcriptional fusion plasmid (pQF-phnW) were grown to exponential phase and exposed to 250 μM t-BOOH. ß-galactosidase activity assays were reported as the mean +/- standard error compared to untreated bacteria. The assays were performed using three independent experiments. The white bars represent untreated bacteria while the gray bars represent t-BOOH treated organisms, respectively.