Fig 6. Time course of t-BOOH degradation by various PA strains.

The rate of PA t-BOOH degradation, an organic hydroperoxide, was investigated using xylenol orange–iron reaction system as described in the materials and methods section. The exponential phase of PAO1 (white bar), oxyR (light gray bar), oxyR/poxyR (dotted bar), phnW::Gm/pphnW (black bar) and phnW::Gm (dark gray bar) cells were used in this study in the present of 200 μM of t-BOOH and measured the rate of degradation of t-BOOH in each cell at difference time points. The percentage of t-BOOH remaining was reported after 12 min of incubation. The experiments were independently repeated at least three times and typical results are shown.