Table 1. Marker expression and fluorescence intensity in oral epithelium biopsies.
(a) Cell surface expression of E-cadherin, EMP1, 5T4, N-cadherin and CD44 in the biopsies used in this study. (b) Fluorescence intensity readings from immunofluorescence microscopy analysis in NT, FEP, LGD, HGD, T1 OSCC and T4 OSCC and their expression within these biopsies (one-way ANOVA).
| A | ||||||||||
| Diagnosis | CDH1$ | EMP1$ | 5T4$ | CDH2$ | CD44$ | |||||
| +ve | -ve | +ve | -ve | +ve | -ve | +ve | -ve | +ve | -ve | |
| Normal Tissue | 12 | 0 | 11 | 1 | 11 | 1 | 1 | 11 | 4 | 8 |
| FEP | 6 | 4 | 8 | 2 | 9 | 1 | 1 | 9 | 1 | 9 |
| LGD | 0 | 20 | 5 | 15 | 12 | 8 | 2 | 18 | 5 | 15 |
| HGD | 0 | 16 | 2 | 14 | 4 | 12 | 4 | 12 | 5 | 11 |
| T1 OSCC | 1 | 4 | 0 | 5 | 3 | 2 | 0 | 5 | 1 | 4 |
| T4 OSCC | 1 | 9 | 1 | 9 | 5 | 5 | 6 | 4 | 0 | 10 |
| LGD-M | 6 | 3 | 6 | 3 | 3 | 6 | 0 | 9 | 2 | 7 |
| HGD-M | 2 | 5 | 4 | 3 | 3 | 4 | 1 | 6 | 0 | 7 |
| T1 OSCC-M | 2 | 3 | 4 | 1 | 2 | 3 | 0 | 5 | 1 | 4 |
| B | ||||||||||
| Diagnosis |
CDH1 Mean FI| |
EMP1 Mean FI| |
5T4 Mean FI| |
CDH2 Mean FI| |
CDH1 vs EMP1* | CDH1 vs 5T4* | CDH1 vs CDH2* | EMP1 vs 5T4* | EMP1 vs CDH2* |
5T4 vs CDH2* |
| NT | 53.1 (1.5/2.8) | 55.5 (6.9/13.5) | 42.9 (7.2/14.2) | 6.3 (5.3/10.3) | ns | ns | 0.0006 | ns | 0.0002 | 0.0003 |
| FEP | 30.4 (6.4/6.4) | 45.7 (9.8/12.8) | 46.2 (7.0/9.1) | 4.6 (3.6/6.7) | ns | ns | ns | ns | 0.017 | 0.004 |
| LGD | 0 | 11.2 (4.5/8.5) | 31.3 (6.5/12.4) | 2.8 (2.8/5.3) | ns | 0.0001 | ns | 0.021 | ns | 0.001 |
| HGD | 0 | 7.4 (5.2/9.8) | 12.2 (5.5/10.5) | 11.6 (5.4/10.2) | ns | ns | ns | ns | ns | ns |
| T1 OSCC | 8.7 (8.7/15.2) | 0 | 26.0 (12.2/21.4) | 0 | ns | ns | ns | ns | ns | ns |
| T4 OSCC | 6.5 (6.5/12.1) | 4.4 (4.4/8.3) | 30.3 (10.9/20.2) | 37.1 (8.7/16.2) | ns | ns | 0.041 | ns | 0.034 | ns |
$Cell surface expression of markers shown.
|Standard error of the mean/Confidence Interval.
*One-Way ANOVA analysis (p-value); FI–relative fluorescence intensity; SEM–standard error of the mean; CI– 95% confidence interval; NT- normal tissue; FEP–fibroepithelial polyp; LGD–low grade dysplasia; HGD–high grade dysplasia; T1 OSCC–T1 stage oral squamous cell carcinoma; T4 OSCC–T4 stage OSCC. CDH1 –E-cadherin; EMP–EMP1; CDH2 –N-cadherin.