(a) eFluor450 dilution by memory (Thy1.1+/+, 1st column) or naive (Thy1.1+/1.2+, 2nd column) C7 cells, and their relative proportion (3rd column) 4 d after co-culture with ESAT63-17 peptide-coated BMDCs (1st row), Mtb-infected BMDCs (2nd row), peptide-coated BMDMs (3rd row), or Mtb-infected BMDMs (4th row). Inset (1st row, 3rd column) indicates input proportions of memory/naive C7 cells. eFluor450 dilution by memory (1st column) and naive (2nd, 3rd columns) C7 cells 5 d after co-culture with (b) peptide-loaded or (c) Mtb-infected, MHC-matched (C57BL10/J) thioglycolate-elicited peritoneal macrophages (TG-PMs) (1st, 2nd columns), or MHC-mismatched (B10.BR) TG-PMs (3rd columns). eFluor450 dilution by in vitro memory C7 cells 5d after culture with Mtb-infected (d) TG-PMs, or (e) BMDCs. (f) Representative CD69 and eFluor450 expression of in vitro memory (top) and naive (bottom) C7 cells 5 d after culture with Mtb-infected TG-PMs. (g) CD69 expression and eFluor450 dilution by vitro memory C7 cells prior to culture with TG-PMs. (h, i) Mtb-infected TG-PMs were cultured for 5 d alone or with (h) in vitro memory or naive C7 cells, or with (i)
in vitro memory or naive P25 cells. The colony-forming units (CFU) were determined on the day of infection (d0) or on d5. As an additional control, in vitro memory or naive P25 cells were also cultured with MHC-mismatched (i.e., B10.BR TG-PMs).