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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Mol Microbiol. 2017 Oct 26;106(6):938–948. doi: 10.1111/mmi.13858

Figure 2. TORC1 is required for nuclear entry of Gln3 in response to nitrogen limitation and downshift.

Figure 2

The tor2-219 (Y511) and tor1 tor2-102 mutant (Y442) cells expressing GLN3-myc were grown at 23°C to early log phase in glutamine (Gln) or proline (Pro) medium. The cultures were divided and incubated for two additional hours with one half at 37°C and the other half at 23°C. A. Cells were transferred from glutamine (Gln) to nitrogen-free (NF) medium. B. Cells were transferred from glutamine (Gln) to proline (Pro) medium. C. Cells were transferred from proline to nitrogen-free medium. Cells were collected before (time 0), 30 and 60 min after the shift. Localization of Gln3-myc in the cells was examined by confocal microscopy. The experiment was repeated three times. Percentages of cells with nuclear staining of Gln3-myc expressed as mean ± SD are shown.