Figure 5. Rapamycin induces normal Mpk1 phosphorylation in proline medium.

Wild type cells (Y1032) grown in glutamine (Gln) or proline (Pro) medium were treated with rapamycin (A) or transferred to nitrogen-free (NF) medium (B) for indicated times. The phosphorylation states of Mpk1 in the treated cells were examined using anti-phospho-MAPK antibody. The levels of Tpd3 were used as loading controls. The experiments were repeated three times and representative data are shown.