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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Mol Microbiol. 2017 Oct 26;106(6):938–948. doi: 10.1111/mmi.13858

Figure 5. Rapamycin induces normal Mpk1 phosphorylation in proline medium.

Figure 5

Wild type cells (Y1032) grown in glutamine (Gln) or proline (Pro) medium were treated with rapamycin (A) or transferred to nitrogen-free (NF) medium (B) for indicated times. The phosphorylation states of Mpk1 in the treated cells were examined using anti-phospho-MAPK antibody. The levels of Tpd3 were used as loading controls. The experiments were repeated three times and representative data are shown.