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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Mol Microbiol. 2017 Oct 26;106(6):938–948. doi: 10.1111/mmi.13858

Figure 6. Npr1 prevents the release of Tap42 from TORC1 in cells grown in proline medium.

Figure 6

Wild type (Y1032) and npr1 deletion (Y1709) cells expressing KOG1-HA grown in glutamine (A) or proline medium (B) were treated with rapamycin (Rap +) or drug vehicle control (Rap −) for 30 min. The same pair of cells grown in glutamine (C) or proline medium (D) were transferred to nitrogen–free (NF) medium for 30 min. The association of Tap42 with Kog1-HA in the cells was examined by immunoprecipitation with anti-Tap42 antibody. The expression levels of Tap42 and Kog1-HA in the cells were examined by western blotting and shown in Supplemental Figure 6. The experiments were repeated three times and representative data are shown.