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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Mol Microbiol. 2017 Oct 26;106(6):938–948. doi: 10.1111/mmi.13858

Figure 7. Npr1 inhibits TORC1 activity.

Figure 7

A. Wild type (Y661) and npr1 deletion (Y238) cells expressing SCH9-HA were grown in glutamine medium and TORC1-dependent phosphorylation of Sch9-HA was assayed by western blotting. B. Cells were shifted from glutamine to proline medium and aliquots of cells were collected before (time 0) and at indicated times after the shift. At the end of 2 hr incubation, the remaining cells were treated with rapamycin for 30 min (Rap). Phosphorylation of Sch9-HA in the cells was assayed by western blotting. The experiments were repeated three times and representative data are shown.