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. 2017 Jun 28;36(1):192–201. doi: 10.1002/jor.23627

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© 2017 The Authors. Journal of Orthopaedic Research® Published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Process of calculating gradients in tissue mineral density (TMD) and trabecular bone volume fraction (BV/TV) at the endplate junction regions. (A) Three‐dimensional μCT data is loaded into custom IDL algorithm, (B and C) algorithm traverses from the dense bony endplate into the deeper vertebral bone, and average values of (D) TMD, and (E) BV/TV are plotted by depth. Differences between peak values (first asterisk) and values 1 mm deeper (second asterisk) were used to assess local gradients in TMD and BV/TV. Note that (B) and (C) are slices in the sagittal and transverse planes, respectively.