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. 2017 Nov 7;7:146–156. doi: 10.1016/j.omtm.2017.11.002

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© 2017 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Comparison of rAAV Vectors Produced by the VV-Ad System and Triple Plasmid Transfection Methods

(A) Capsid composition of rAAV vectors produced by VV-Ad and triple plasmid transfection (Tri) methods. rAAV vectors (∼1 × 10¹⁰ particles) purified after two rounds of CsCl ultracentrifuge were electrophoresed and visualized by silver staining. (B) rcAAV contamination in rAAV vectors was determined by measuring the percentage of Rep78 containing genomes in total rAAV genomes. (C) Transduction activity of dsAAV2-CB-EGFP vectors in vivo after intravitreal injection. Confocal images of flat-mounted retinas showing EGFP⁺ RGCs and Tuj1 immunostaining, and EGFP⁺ axons in optic nerve (n = 5, *p < 0.05).