Table 1.
rAAV Yield of the Optimized VV-Ad System
| Vector Name | Vector Size (kb) | Corresponding Ad/AAV Name | Vector Yield |
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|---|---|---|---|---|---|---|---|---|---|
| Adherent Culture (vg/Cell) | Suspension Culture |
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| Low Cell Density (l × 106 Cells/mL) |
Modest Cell Density (2.5 × 106 Cells/mL) |
High Cell Density (5 × 106 Cells/mL) |
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| vg/Cell | vg/L | vg/Cell | vg/L | vg/Cell | vg/L | ||||
| ssAAV2-CMV-EGFP | 4.0 | Ad/AAV-CMV-EGFP | 2.37 ± 0.7 × 105 | 2.01 ± 0.42 × l05 | 2.01 ± 0.42 × l014 | 1.79 ± 0.43 × l05 | 4.48 ± 1.08 × l014 | 1.95 ± 0.22 × 105 | 9.75 ± 1.10 × l014 |
| ssAAV2-CMV-LacZ | 4.7 | Ad/AAV-CMV-lacZ | 0.96 ± 0.14 × 105 | 0.59 ± 0.04 × l05 | 0.59 ± 0.04 × l014 | – | – | – | – |
| dsAAV2-CB-E GFP | 2.1 | Ad/dsAAV-CB-EGFP | 1.78 ± 0.04 × l05 | 1.60 ± 0.01 × l05 | 1.60 ± 0.01 × l014 | – | – | – | – |
| dsAAV2-CB-G luc | 1.8 | Ad/dsAAV-CB-Glue | 1.54 ± 0.27 × l05 | 1.17 ± 0.04 × l05 | 1.17 ± 0.04 × l014 | 1.09 ± 0.13 × 105 | 2.72 ± 0.32 × l014 | – | – |
| ssAAV2-CB-h AAT | 2.6 | Ad/AAV-CB-A AT | 4.04 ± 0.90 × l05 | 2.76 ± 0.11 × l05 | 2.76 ± 0.11 × l014 | – | – | 2.03 ± 0.43 × l05 | 10.2 ± 2.15 × l014 |
Suspension QW158-7 cells were infected with the Ad/AAV hybrid (MOI = 50) and VW22-PM (MOI = 2). The titer of rAAV vectors was measured by qPCR at 48 hr post-VW22-PM infection.