Skip to main content
. 2017 Oct 16;191(1):19–31. doi: 10.1111/cei.13046

Figure 3.

Figure 3

Ex‐vivo study of histone deacetylase (HDA)C6 inhibition reduces interferon (IFN)‐α production from plasmacytoid dendritic cells (pDCs). (a) The viability of sorted pDCs from bone marrow after treatment with different concentrations of the selective HDAC6 inhibitor (ACY‐738) for 24 h. (b) IFN‐α production of treated pDCs with HDAC6 inhibitor from bone marrow upon cytosine–phosphate–guanosine (CpG) stimulation for 24 h. (c) The viability of sorted pDC from bone marrow after treatment with two different concentration of negative control small‐interfering RNA (siRNA) and HDAC6 siRNA, respectively, for 24 h. (d) IFN‐α production of treated pDCs with siRNA from bone marrow upon CpG stimulation for 24 h. *P < 0·05; **P < 0·01; ***P < 0·001; ****P < 0·0001; one‐way analysis of variance (anova); n = 3.