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. 2017 Oct 5;191(1):107–115. doi: 10.1111/cei.13048

Figure 2.

Figure 2

Lipopolysaccharide (LPS)‐induced dendritic cell‐specific intercellular adhesion molecule 3‐grabbing non‐integrin 1 (DC‐SIGN) expression. Human kidney 2 (HK‐2) cells were incubated with 100 ng/ml LPS with increasing time intervals (0, 6, 12 and 24 h) or with increasing doses (0, 1, 10 and 100 ng/ml) for 12 h. DC‐SIGN expression levels were detected by real‐time polymerase chain reaction (PCR) (a,b) and Western blotting (c,d), which were quantified by densitometry in three independent experiments as relative units (DC‐SIGN/α‐tubulin). The data are expressed as the mean ± standard deviation (s.d.) from three independent tests. *P < 0·05; **P < 0·01 compared with LPS untreated HK‐2 cells.