Figure 6.

Hepatic fibrosis is increased in hCYP2E1 mice after CCl₄ injury. Liver fibrosis induced by CCl₄ was assessed in Wt and hCYP2E1 mice. Mice receiving corn oil were used as controls. (A) CCl₄‐induced liver injury was evaluated by serum ALT level. (B) Immunoblotting and mRNA expression level of CYP2E1 in liver from both corn oil and CCl₄ groups. (C) Representative images of Sirius Red and immunohistochemistry staining of Desmin, α‐SMA, 4‐HNE, and F4/80 are presented with quantification. (D) mRNA levels of fibrogenic genes were measured in Wt and hCYP2E1 mice after CCl₄ injury or oil application. Data are shown as the fold change of mRNA induction compared with Wt mice receiving corn oil. The P value was measured between Wt and hCYP2E1 mice after CCl₄ injury; the data were shown as mean ± SEM; *P < 0.05.