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. 2017 Oct 25;1(10):1099–1109. doi: 10.1002/hep4.1114

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© 2017 The Authors. Hepatology Communications published by Wiley Periodicals, Inc., on behalf of the American Association for the Study of Liver Diseases.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Reversal of EMT induced by periostin knockdown in HuH28 cells. HuH28 cells were transfected with siRNA targeting human periostin or a nontargeting scrambled control. (A) Knockdown of periostin was confirmed using real‐time PCR (n = 6). The periostin concentration in culture media after 7 days of transfection was quantified using ELISA (n = 3). (B) Gene expression of epithelial (E‐cadherin and CK19) and mesenchymal (N‐cadherin, vimentin, and fibronectin) markers was analyzed using real‐time PCR after 7 days of siRNA transfection. (C) Protein expression of periostin and EMT‐related markers was determined using immunoblotting. Data are expressed as the mean ± SEM; *P < 0.05, **P < 0.01. Abbreviations: GAPDH, glyceraldehyde‐3 phosphate dehydrogenase; Scr, scrambled control; siPN, siRNA targeting human periostin.