Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 13;1(7):675–690. doi: 10.1002/hep4.1066

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 The Authors. Hepatology Communications published by Wiley Periodicals, Inc., on behalf of the American Association for the Study of Liver Diseases.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

The amounts of total and cytochrome P450 proteins in circulating EVs were increased in human patients with alcoholism. (A) Analysis of the size of circulating EVs isolated from the sera of healthy controls (n = 9) who never drank alcohol due to religious reasons and patients with alcoholism (n = 14) with hepatitis. The inset figures represent transmission electron microscopy images. (B) EV size was determined by a Nanoparticle Tracking analysis (Nanosight).18 (C) Total number of EVs in the sera of healthy controls or patients with alcoholism was measured by Nanosight. (D) Analysis of the total protein amounts in circulating EVs isolated from the sera of healthy controls or patients with alcoholism. (E) Detection of the cytochrome P450 proteins (i.e., CYP2E1, CYP2A6, CYP1A1/2, CYP4A, and CYP4B) in circulating EVs from the different groups by immunoblot analyses, as indicated. CD63 was used as a loading control for the same amounts of total EV proteins in all samples. Densitometric quantitation of the immunoblots for CYP2E1, CYP2A6, CYP1A1/2, CYP4A, and CYP4 relative to CD63 (n = 4/group) is shown. *P < 0.05, **P < 0.01. Data are shown as means ± SD.