Skip to main content
. 2017 Jul 13;1(7):675–690. doi: 10.1002/hep4.1066

Figure 3.

Figure 3

PUFA supplementation prevented the total protein amounts and CYP proteins in circulating EVs. (A) Analysis of the size of circulating EVs isolated from Long‐Evans rat plasma of the BC, BE, PC, or PE group performed by Nanosight.19 (B) Total number of EVs in plasma of the BC, BE, PC, and PE groups was measured by Nanosight. (C) Analysis of the total protein amounts in circulating EVs isolated from rat plasma of the BC, BE, PC, or PE group. (D) Detection of the cytochrome P450 proteins (e.g., CYP2E1, CYP2A3, CYP1A1/2, CYP4A, and CYP4B) in circulating EVs from the different groups by immunoblot analyses, as indicated. Densitometric quantitation of the immunoblots for EV CYP proteins relative to CD63, used as a loading control for the same amounts of total EV proteins, is shown. n = 3/group. (E) Detection of the CYP proteins in liver lysates from the different groups by immunoblot analyses, as indicated. Densitometric quantitation of the immunoblots for CYP proteins relative to β‐actin, used as a loading control, is shown. n = 4/group. *P < 0.05, **P < 0.01. Data are shown as means ± SD.