Table 3.
RESULTS OF METABOLITE SET ENRICHMENT ANALYSIS
| Metabolic Pathway | Total Metabolites | Detected Metabolites | Selected Metabolites | P Value (Fisher's Exact Test)a | Q Value (BH Method) |
|---|---|---|---|---|---|
| Tricarboxylic acid cycle | 13 | 6 | 3 | 0.0024 | 0.0636 |
| Methionine metabolism | 25 | 3 | 1 | 0.1743 | 1.0000 |
| Glutamic acid and glutamine metabolism | 11 | 5 | 1 | 0.2759 | 1.0000 |
| Shikimic acid metabolism | 27 | 5 | 1 | 0.2759 | 1.0000 |
| Tyrosine metabolism | 76 | 5 | 1 | 0.2759 | 1.0000 |
| Glycine, serine and threonine metabolism | 42 | 17 | 1 | 0.6917 | 1.0000 |
| Glycolysis/gluconeogenesis | 11 | 3 | 0 | 1.0000 | 1.0000 |
| Pentose phosphate pathway | 10 | 2 | 0 | 1.0000 | 1.0000 |
| Alanine, aspartic acid, and asparagine metabolism | 11 | 6 | 0 | 1.0000 | 1.0000 |
| Lysine metabolism | 55 | 6 | 0 | 1.0000 | 1.0000 |
| Valine, leucine, and isoleucine metabolism | 22 | 8 | 0 | 1.0000 | 1.0000 |
| Cysteine metabolism | 30 | 11 | 0 | 1.0000 | 1.0000 |
| Histidine metabolism | 39 | 8 | 0 | 1.0000 | 1.0000 |
| Urea cycle | 23 | 7 | 0 | 1.0000 | 1.0000 |
| Proline metabolism | 22 | 5 | 0 | 1.0000 | 1.0000 |
| Polyamine metabolism | 20 | 3 | 0 | 1.0000 | 1.0000 |
| Tryptophan metabolism | 80 | 4 | 0 | 1.0000 | 1.0000 |
| Beta‐alanine metabolism | 31 | 7 | 0 | 1.0000 | 1.0000 |
| Taurine and hypotaurine metabolism | 20 | 6 | 0 | 1.0000 | 1.0000 |
| Creatine metabolism | 11 | 8 | 0 | 1.0000 | 1.0000 |
| Purine metabolism | 87 | 12 | 0 | 1.0000 | 1.0000 |
| Pyrimidine metabolism | 56 | 8 | 0 | 1.0000 | 1.0000 |
| Ribonucleotide metabolism | 18 | 6 | 0 | 1.0000 | 1.0000 |
| Deoxyribonucleotide metabolism | 19 | 0 | 0 | 1.0000 | 1.0000 |
| Conjugated bile acid metabolism | 5 | 1 | 0 | 1.0000 | 1.0000 |
| Nicotinic acid metabolism | 43 | 6 | 0 | 1.0000 | 1.0000 |
a
All significantly different metabolites found between the high‐HIS and low‐HIS groups by Welch's t test were later analyzed by the metabolite set enrichment analysis, which identified the tricarboxylic acid cycle pathway as the only significant pathway. P values in bold indicate a significant difference.
Abbreviation: BH method, Benjamini and Hochberg method.