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. 2017 Mar 27;1(3):215–229. doi: 10.1002/hep4.1026

Figure 8.

Figure 8

Regulation of Afp gene induction in primary hepatocytes by Jagged1/Notch2 signaling. (A) Primary hepatocytes isolated from wild‐type mice were cocultured with either quiescent or activated HSC in a mixed condition in the presence of DMSO or 10 μM GSI for 36 hours. (B) Primary hepatocytes isolated from wild‐type mice were cocultured with HSC derived from either control or Jag1 cKO mice for 36 hours. (C) Primary hepatocytes isolated from either wild‐type or Notch2 cKO mice were cocultured with the wild‐type HSC for 36 hours. Afp gene expression levels were quantified by real‐time RT‐PCR. The values indicate the means ± SD from four samples in each group and represent two or three independent experiments. An asterisk indicates that the difference between groups was statistically significant (P < 0.01). Abbreviations: DMSO, dimethyl sulfoxide; GSI, gamma secretase inhibitor IX; N.S., not significant.