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. 2017 Apr 19;1(3):230–247. doi: 10.1002/hep4.1032

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© 2017 The Authors. Hepatology Communications published by Wiley Periodicals, Inc., on behalf of the American Association for the Study of Liver Diseases.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Histopathological analysis of hepatocytes in MAN1A1 and MAN1C1 transgenic fish at 3, 5, 7, 9, and 11 months of age. (A) Hematoxylin and eosin staining of liver sections from the TG1 and TG3 independent lines of MAN1A1 and MAN1C1(TG1) transgenic fish. Hematoxylin and eosin staining of the liver sections from MAN1A1 transgenic fish showed various pathological features, such as steatosis, inflammation, hyperplasia, dysplasia, and HCC. The MAN1C1 transgenic fish and EGFP‐mCherry control fish revealed normal phenotypes. The images were taken at magnification ×400, and scale shown is for 30 μm. (B) Statistical analysis of MAN1A1 (TG1), (C) MAN1A1 (TG3), (D) MAN1C1 (TG1), and (E) Tg(fabp10a:EGFP‐mCherry) is shown; n = 5 for each stage of transgenic fish.