Fig. 4.

Overexpression of PSAT1 promoted the proliferation of ER-negative breast cancer cells. a Overexpression of PSAT1 in BT-549 cells was analyzed by WB. β-tubulin was used as a loading control. b The proliferation of BT-549 cells with stably up-regulated PSAT1 were tested by CCK-8 assay. c Overexpression of PSAT1 enhanced the colony formation ability of BT-549 cells. The values of the vector-control cells were normalized to 1. In (B) and (C), the results are expressed as the mean ± SD; n = 3. d The cell cycle was analyzed in BT-549 cells with stable overexpression of PSAT1 by flow cytometry. **p < 0.01. ****p < 0.0001