. Author manuscript; available in PMC: 2018 Sep 11.

Published in final edited form as: Curr Protoc Pharmacol. 2017 Sep 11;78:11.20.1–11.20.24. doi: 10.1002/cpph.25

Table 1.

Key parts for custom microscope assembly. Additional standard components available from Thorlabs will be needed for mounting the lasers, lenses, and mirrors, including posts (e.g. TR4), post holders (e.g. PH4), and base plates (e.g. BA1S).

Part	Vendor	Part number
camera	Hamamatsu	ORCA-Flash4.0 sCMOS
60× TIRF oil objective	Olympus	APON 60XOTIRF
inverted microscope	Olympus	IX-71 (or similar)
red laser	Coherent	OBIS 637 LX 140 mW
blue laser	Coherent	OBIS 488 LX 50 mW
emission dichroic, d2	Semrock	Di03-R405/488/561/635-t1-25×36
QuasAr emission filter	Semrock	FF02-736/128-25
GFP emission filter	Semrock	FF02-525/40-25
blue laser excitation filter	Semrock	FF01-488/10-25
red laser excitation filter	Semrock	FF01-637-7-25
beam-combining dichroic, d1	Semrock	LM01-503-25
mounted mirrors (M1 – M2)	Thorlabs	KM100-E02
10× beam expander	Thorlabs	GBE10-A
DAQ card	National Instruments	USB-6343