Table 1. List of oligonucleotides used for mutagenesis.
| Residue
change(s) |
Primer sequence (5’ → 3’); F: forward; R: reverse. |
|---|---|
| T397S |
F: AAGGCCTTCAGGCACTGAGCAAGAATGTTTTTCTG
R: CAGAAAAACATTCTTGCTCAGTGCCTGAAGGCCTT |
| F404L |
F: CATAATATTCTGCAGGTAACAGAAGGCCTTCAGGC
R: GCCTGAAGGCCTTCTGTTACCTGCAGAATATTATG |
| P405T |
F: GCCTGAAGGCCTTCTGTTTACTGCAGAATATTATGTTAG
R: CTAACATAATATTCTGCAGTAAACAGAAGGCCTTCAGGC |
| F404A/P405A |
F: ACAGTGCCTGAAGGCCTTCTGGCTGCTGCAGAATATTATGTTAGAAC
R: GTTCTAACATAATATTCTGCAGCAGCCAGAAGGCCTTCAGGCACTGT |
| Y408H |
F: CGTGTTGTTCTAACATAATGTTCTGCAGGAAACAGAAGGC
R: GCCTTCTGTTTCCTGCAGAACATTATGTTAGAACAACACG |
| E407A/Y408A |
F: GAAGGCCTTCTGTTTCCTGCAGCAGCTTATGTTAGAACAACACGAAGC
R: GCTTCGTGTTGTTCTAACATAAGCTGCTGCAGGAAACAGAAGGCCTTC |
| V410G |
F: GTTTCCTGCAGAATATTATGGTAGAACAACACGAAGCATG
R: CATGCTTCGTGTTGTTCTACCATAATATTCTGCAGGAAAC |
| T413S |
F: CAGAATATTATGTTAGAACATCACGAAGCATGTCCAATTG
R: CAATTGGACATGCTTCGTGATGTTCTAACATAATATTCTG |
| V410P/T413P |
F: GCCTTCTGTTTCCTGCAGAATATTATCCTAGAACACCACGAAGCATGTCC
R: GGACATGCTTCGTGGTGTTCTAGGATAATATTCTGCAGGAAACAGAAGGC |
| S417Y |
F: GAACAACACGAAGCATGTACAATTGCCAGAGGAAAG
R: CTTTCCTCTGGCAATTGTACATGCTTCGTGTTGTTC |
| N418A/C419I |
F: TAGAACAACACGAAGCATGTCCGCTATCCAGAGGAAAGTAGCCGTGGAG
R: CTCCACGGCTACTTTCCTCTGGATAGCGGACATGCTTCGTGTTGTTCTA |
| C419G |
F: AACAACACGAAGCATGTCCAATGGCCAGAGGAAAG
R: CTTTCCTCTGGCCATTGGACATGCTTCGTGTTGTT |
| C419P |
F: AGAACAACACGAAGCATGTCCAATCCCCAGAGGAAAGTAGC
R: GCTACTTTCCTCTGGGGATTGGACATGCTTCGTGTTGTTCT |
| V425M |
F: GCCAGAGGAAAGTAGCCATGGAGGCTGTCATTCAG
R: CTGAATGACAGCCTCCATGGCTACTTTCCTCTGGC |
| A427P/I429P |
F: GAGGAAAGTAGCCGTGGAGCCTGTCCCTCAGAGTCATTTGGATGTC
R: GACATCCAAATGACTCTGAGGGACAGGCTCCACGGCTACTTTCCTC |