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. 2016 Nov 3;6:9–15. doi: 10.1016/j.ensci.2016.11.002

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© 2016 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Intracellular Ca^2 + measurements. Intracellular Ca^2 + release was evaluated by monitoring Fluo4 fluorescence. A. Background (before stimulation) fluorescence is shown in the upper panels and peak (after stimulation) fluorescence is shown in the lower panels. Scale bar = 100 μm. B. Time course of Ca^2 + release responses. The data represent the average of 15 individual cells from the same dish. MyoD (+): MyoD-transduced fibroblasts, MyoD (−): normal fibroblasts. C. The difference between the peak and background intensity of fluorescence. n = 15. MyoD (+): MyoD-transduced fibroblasts, MyoD (−): normal fibroblasts.