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. 2016 Nov 3;6:9–15. doi: 10.1016/j.ensci.2016.11.002

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© 2016 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 4 — Analysis of cell migration of Ad.CAGMiG-transduced cells using time-lapse microscopy. A. Migratory path of Ad.CAGMiG-transduced primary human skin fibroblasts is analyzed using time-lapse microscopy. Fifteen individual cells were marked at random and individually tracked as all shown (yellow lines). The total migration distances of cells #11–15 are indicated in the upper left corner. Scale bar = 100 μm. B. Average velocity (μm/h) of 15 individual cells Ad.CAGMiG- and Ad.CAGEGFP-transduced primary human skin fibroblasts and non-transduced mouse myoblasts (wild type) calculated using BZ-H1M software (Keyence). The velocity of Ad.CAGMiG-transduced cells was significantly higher compared to the other cells. Data are expressed as the mean ± SEM; n = 15; *P < 0.001 **P < 0.001.