Fig. 2.

Comparison of AR and PSA expression and aggressiveness in LNCaP, PDB and MDB. a Immunolocalization of AR (top) and PSA (bottom) in the three cell lines. The scale bars correspond to 100 μm and 20 μm (inset). b WB analysis of AR and PSA gene expression in the three cell lines. Samples were probed with antibodies against AR and PSA. c Evaluation of the capability of the three cell lines to invade Matrigel in response to human fibroblast-conditioned medium (*p < 0.05; *** p < 0.001). d In vivo experiments to evaluate tumor growth in NOD SCID male mice by LNCaP, PDB and MDB cells. Twelve animals were inoculated subcute with each cell line