Fig. 3.

Characterization of phosphorylation and transcriptional activity of AR in LNCaP, PDB and MDB. a The overall degree of AR phosphorylation was evaluated for total proteins extracted from LNCaP, PDB and MDB by Phos-tag SDS-PAGE technique and immunoblotted for AR (upper panel). The relative amounts of phosphorylated AR (p-AR) were obtained by normalizing the integrated optical density by the relative amounts of immunoreactive AR bands (p-AR + AR) for each sample (lower panel). The bars represent the mean ± SE of three experiments. b AR transcriptional activity determined by the luciferase activity assay. c Cell viability of LNCaP, PDB and MDB cells incubated for 120 h with different concentrations of enzalutamide (ENZA)