Fig. 4.

Particle uptake by LPS-primed BMDMs after chasing with TiO₂ +/− peptidoglycan or MDP. BMDMs from WT (a) and Nod2 ^m/m (b) mice were pre-stimulated for 3 h with LPS (10 ng/mL). Then BMDMs were incubated for 3 h with the indicated concentrations of TiO₂ particles suspended in TCM alone (TCM), TCM + 10 μg/mL MDP (MDP), or TCM + 10 μg/mL peptidoglycan (PGN). Cells were stained with PI and F4/80 antibody for murine macrophages and analysed with flow cytometry, and median SSC intensities of PI⁻F4/80⁺ cells were recorded. Data represent mean ± SD from two independent experiments with three replicates each, n = 6