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. 2017 Jun 19;1(1):e000056. doi: 10.1136/bmjophth-2016-000056

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This is an Open Access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 4.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. See: http://creativecommons.org/licenses/by/4.0/

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(1) Representative ocular photomicrographs of groups A, C57BL/6 untreated; B, CCL2^−/− untreated; C, CCL2^−/− treated with ω-3+ω-6; and D, CCL2^−/− treated with ω-3. Cryosections were stained for cone arrestin (red), rhodopsin (green) and TO-PRO-3 iodide (blue) for nuclear staining. Samples were examined by a Leica TCS SP5 confocal microscope (original magnification 100×, scale bar=50 µm). Arrow indicates retinal ONL. (2) Measurements of the ONL were performed at different fields around the entire retinal section (centre, middle and periphery). ImageJ software was used to calculate the ONL thickness, and the mean ONL thickness of the entire retina was compared among the different groups. Data represent the mean±SEM (n=5).

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