Table 1.
List of primers used in the SYBR green-based qRT-PCR analysis
| Gene target | Primer sequence (5′–3′) | Product length (bp) | Reference | |
|---|---|---|---|---|
| GAPDH | F | CCTGCACCACCAACTGCTTG | 222 | [62] |
| R | TTGAGCTCAGGGATGACCTTG | |||
| IFN-γ | F | GTCTCCTTCTACTTCAAACT | 253 | [63] |
| R | ATTCTGACTTCTCTTCCGCT | |||
| TGF-β | F | CGTGCTAATGGTGGAATAC | 208 | Present study |
| R | GCCAGGAATTGTTGCTATA | |||
| IL-1β | F | CTAGCCCATGTGTGCTGAAG | 59 | [62] |
| R | CCTTTACTTGGCTCTTCACC | |||
| IL-4 | F | CAGCATGGAGCTGCCT | 177 | [64] |
| R | ACAGAACAGGTCTTGCTTGC | |||
| IL-6 | F | CTGCAATGAGAAAGGAGATA | 191 | [63] |
| R | GGTAGTCCAGGTATATCTGA | |||
| IL-10 | F | CTGTGCCTCTCCCCTAGAGT | 236 | [62] |
| R | GCAGCTAGCTCCACAAGGAA | |||
| IL-12B | F | CAGGGACATCATCAAACCAG | 213 | [63] |
| R | CTTGTGGCATGT GACTTTGG | |||
| IL-13 | F | AGAACCAGAAGGTGCCGCT | 50 | [65] |
| R | GGTTGAGGCTCCACACCATG | |||
| IL-17A | F | CTACAGTGAACTGGAAGGAAC | 554 | Present study |
| R | AAAAGGGGCTGGGTCT | |||
| T-bet | F | CCTGGACCCAACTGTCAACT | 171 | [66] |
| R | GAAACTCGGCCTCATAGCTG | |||
| GATA-3 | F | GATCAAGCCCAAGCGAAGG | 124 | Present study |
| R | CCGCAGGCATTGCAGACA | |||
| Foxp3 | F | GACAGCACCCTTTCGACTGT | 191 | [66] |
| R | CTCCAGAGATTGCACCACCT | |||
| ROR-γτ | F | CTACAGTGAACTGGAAGGAAC | 554 | Present study |
| R | AAAAGGGGCTGGGTCT | |||
Abbreviations: F forward primer, R reverse primer