Fig. 2.

Total protein analysis by the Dumbroff method of non-covalent binding to filter paper followed by washing of non-proteinaceous components with methanol prior to staining with 0.1 g CBBR₂₅₀ in 40% methanol and 10% acetic acid. Plasma samples were mixed 1:1 with 2 × tris sample buffer for SDS-PAGE and then diluted a further 10 fold in 2 × tris sample buffer (dF 20) into the linear BSA standard range